FAQ1: Does the volume of T1 has to be exact?
A large volume is needed. It is important that the tip of the straw is moved when plunged in the medium and the dish is shaken at the same time. This to avoid decreasing temperature around the embryo (avoid recrystallization and aggregation of lethal ice crystals).
FAQ2: Do reagents need to be warmed in a CO2 incubator?
No, only to 37°C in a standard incubator.