Sil-Select Stock

Sperm preparation media – stock solution

Sil-Select Stock is our home made stock solution for preparing density gradient system for semen preparation. Sil-Select Stock is an isotonic solution of silane-coated silica particles in a chemically defined, balanced salts solution with HEPES (pH buffer). Sil-Select Stock can be used in combination with IUI, IVF and ICSI. The density of Sil-Select Stock is approximately 1.12g/mL.

Extensive testing during the design of the product has shown it to be non-cytotoxic and non-irritant with a non-significant allergenic potential.

Sil-Select Stock is manufactured from raw colloidal silica, so we have full control over each step in the production process. We have developed a very accurate purification system which results in a very clean product with endotoxin levels below 0.5EU/mL.

Information about the composition of the product can be found in the material safety data sheet.

Regulatory

Europe: CE-marked – USA: US FDA cleared
Canada: Health Canada License – Brazil: registered

Product order codes
SIS100 : Sil-Select Stock – 100mL
SIS500 : Sil-Select Stock – 500mL
SIS100G : Sil-Select Stock with gentamicin – 100mL

Product specifications

pH	7.2 – 7.9 (7.2-7.6 at product release)
Osmolality	300-330mOsm/kg
Sterility	Sterile
Endotoxine	< 0.5 EU/mL
Sperm Survival test	≥ 80% motility recovery after 4 hours exposure ≥ 75% motility recovery after 24 hours exposure
Density	1.1150-1.1250g/mL
Viscosity in cP (25°C)	<1.75cP
Shelf life	18 months from date of produce

Key components

Component	Benefit
HEPES (18 mM)	HEPES stabilizes the pH under air (Clark and Swain 2014), therefore CO₂ incubation is not required.
Earle’s Balanced Salt Solution (EBSS)	The composition of the salt solution results in an optimum pH and osmolality environment for sperm cells.
Silane coated colloidal silica	Density gradient media have been optimized for maximum efficiency in the selection of spermatozoa for use in ART. Their density is directly linked with their functionality and depends on the % silanized colloidal silica particles added in the media.

Product literature

Dal Canto, MB.; Mignini Renzini, M.; Brambillasca, F.; et al., IVM–the first choice for IVF in Italy., Reprod. Biomed. Online (2003),Vol.13,No.2,pp.159-65

Ha, JH.; Oum, KB.; Chung, HM.; et al., Comparison of Sperm Motility, Recovery Rate, and Fertilization Rate using Three Different Sperm Preparation Methods: Swim-up, Percoll, Sil-Select., Korean J Fertil. Steril. (26),Vol.2,pp.257-263

Jarupoonpol, S. Choktanasiri, W.; Wongkurlarb, A.; et al., Sperm Motility and DNA integrity affected by different g-forces in the preparation of sperm in uterine specimens., Andrologia (46),Vol.4,pp.391-398

Jedrzejczak P., Rzepczynska I., Taszarek-Hauke G., Pawelczyk L., Effect of sperm subpopulation’s kinetics on human fertilization in vitro, Archives of Andrology (2005),Vol.51,pp.185-193

Trokoudes, KM.; Minbattiwalla, MB.; Kalogirou, L.; et al., Controlled natural cycle IVF with antagonist use and blastocyst transfer., Reprod. Biomed. Online (2005),Vol.11,No.6,pp.685-9

Literature about components

Frequently Asked Questions Certificate Of Analysis

Sperm Processing