GAINTM medium

Intended use:

GAINTM medium is a single-step cell culture medium for use with human embryos and gametes. GAIN™ medium can be used in all the procedures below:

  • For semen washing and intra uterine insemination (IUI).
  • For oocytes handling/incubation in preparation of, or during fertilization by in vitro fertilization (IVF)/ intra cytoplasmatic sperm injection (ICSI).
  • For embryo culture from day 1 to expanded blastocyst stage.
  • For embryo transfer (ET).

GAINTM medium is ready-to-use.


GAINTM medium is a bicarbonate-buffered balanced salt solution, supplemented with 10mg/l gentamicin (medicinal substance) and 3.5g/l human serum albumin (medicinal substance derived from human blood plasma) and phenol red.

Further information on the composition of the medium can be found in the material safety data sheet.


  • Europe: CE-marked (MDR, Notified Body number 2797)
  • Brazil: Registered
  • Other regions: Information available upon request

Product order codes:

GAIN010 : 5 x 10ml – GAINTM medium
GAIN020 : 5 x 20ml – GAINTM medium

pH 37°C – 5% CO2 7.20-7.45
Osmolality 270-290mOsm/kg
Sterility by the current Ph. Eur. 2.6.1./ USP <71> No growth
Endotoxin < 0.25 EU/mL
Mouse embryo assay (% blastocysts after 96 hours culture in test medium) ≥ 80%
Human sperm survival assay (% motility compared with control after 24 hours exposure to test medium) ≥ 80%
Concentration human serum albumin 2.8-4.2 g/l
Shelf life 12 months from date of production

Oocyte Sperm Processing Fertilization Media Embryo Transfer Insemination

GAIN medium is a single-step cell culture medium that can be used up to blastocyst stage

Instructions For Use
Summary of safety and clinical performance
Material Safety Data Sheet