Sil-Select Plus is our home made new, improved and ready-to-use gradient system for semen preparation. The following gradients are available:
– Sil-Select Plus Upper layer (45%)
– Sil-Select Plus 80%
– Sil-Select Plus Lower layer (90%)
Sil-Select Plus gradients consist of silane-coated colloidal sicila particles suspended in HEPES-buffered Earle’s balanced salts solution, and as from batch FP18SIP20 supplemented with 0.4 – 2.2g/l human serum albumin.
Extensive testing during the design of the product has shown it to be non-cytotoxic and non-irritant with a non-significant allergenic potential.
Sil-Select Plus is produced from raw colloidal silica, so we have full control over each step in the production process. We have developed a very accurate purification system which results in a very clean product with endotoxin levels below 0.5EU/mL.
The Sil-Select Plus density gradient system (45%-90%) consists of a Sil-Select Plus Upper Layer (45%) and a Sil-Select Plus Lower Layer (90%).
We also offer Sil-Select Plus in an 80% dilution for use in IVF procedures.
Information about the composition of the products can be found in the material safety data sheet.
Europe: CE-marked – Canada: Health Canada License – Brazil: registered
Product order codes
SIP008 : Sil-Select Plus – 8 preparations
SIP016 : Sil-Select-Plus – 16 preparations
SIP050 : Sil-Select Plus – 2 x 50mL
SIP100 : Sil-Select Plus – 2 x 100mL
SIP80_100 : Sil-Select Plus 80% – 100mL
HEPES stabilizes the pH under air (Clark and Swain 2014), therefore CO2 incubation is not required.
Earle’s Balanced Salt Solution (EBSS)
The composition of the salt solution results in an optimum pH and osmolality environment for sperm cells.
Silane coated colloidal silica
Density gradient media have been optimized for maximum efficiency in the selection of spermatozoa for use in ART. Their density is directly linked to their functionality and depends on the percentage silanized colloidal silica particles added to the media.
HSA (0.4 – 2.2 g/L)
Sil-Select Plus contains 0.4 – 2.2 g/L Human Serum Albumin (HSA) to optimize medium performances. HSA is universally added to most ART media because it is widely considered to be of benefit.
The role of albumin in ART media is extensive, including:
– Stabilization of the cell membrane of the embryo in the medium (Malda, et al. 2008).
– Inhibition of lipid peroxidation that can be damaging to sperm (Alvarez en Storey 1995).
– Carrier and source of essential molecules needed by the embryo (Malda, et al. 2008)
– Detoxification by binding waste products from cell metabolism (Blake, et al. 2004)
– Facilitating gamete/embryo manipulation by preventing adsorption to the surface through saturation of potential binding sites (Blake, et al. 2004)
Dal Canto, MB.; Mignini Renzini, M.; Brambillasca, F.; et al., IVM–the first choice for IVF in Italy., Reprod. Biomed. Online (2003),Vol.13,No.2,pp.159-65
Ha, JH.; Oum, KB.; Chung, HM.; et al., Comparison of Sperm Motility, Recovery Rate, and Fertilization Rate using Three Different Sperm Preparation Methods: Swim-up, Percoll, Sil-Select., Korean J Fertil. Steril. (26),Vol.2,pp.257-263
Jarupoonpol, S. Choktanasiri, W.; Wongkurlarb, A.; et al., Sperm Motility and DNA integrity affected by different g-forces in the preparation of sperm in uterine specimens., Andrologia (46),Vol.4,pp.391-398
Jedrzejczak P., Rzepczynska I., Taszarek-Hauke G., Pawelczyk L., Effect of sperm subpopulation’s kinetics on human fertilization in vitro, Archives of Andrology (2005),Vol.51,pp.185-193
Trokoudes, KM.; Minbattiwalla, MB.; Kalogirou, L.; et al., Controlled natural cycle IVF with antagonist use and blastocyst transfer., Reprod. Biomed. Online (2005),Vol.11,No.6,pp.685-9
Literature about components
Alvarez, JG., and BT. Storey, Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa., Mol Reprod Dev. (1995),Vol.42,No.3,pp.334-46
Blake, D., P. Svalander, M. Jin, C. et al., Protein Supplementation of Human IVF Culture Media., Journal of Assisted Reproduction and Genetics (2002),Vol.19,No.3,pp.137-143
Clark, N.A. and Swain, J.E., Buffering systems in IVF., Culture media, Solutions, and Systems in Human ART, by P. Quinn (2014),pp.30-46
Malda, J. et al., Cell Nutrition, In Tissue engineering, by C. Van Blitterswijk. London, UK: Academia Press, Elsevier, (2008),pp.327-362