FertiCultTM Flushing medium

Intended use

FertiCultTM Flushing medium is intended for in vitro procedures involving human gametes (sperm and oocytes), including washing of gametes, sperm swim-up procedures, intra-uterine insemination (IUI) of the spermatozoa and sperm injection during intracytoplasmic sperm injection (ICSI). FertiCultTM Flushing medium can also be used for human embryo washing and holding, and for embryo transfer (ET) in the uterine cavity.

FertiCultTM Flushig medium is ready-to-use.

Composition
FertiCultTM Flushing medium is a ready-to-use HEPES buffered medium which also contains bicarbonate, physiologic salts, glucose, lactate, pyruvate and human serum albumin (4.0g/l, medicinal substance derived from human blood plasma). The product is also available with phenol red and/or gentamicin (10mg/l, medicinal substance).

Further information on the composition of the media can be found in the material safety data sheet.

Regulatory

  • Europe: CE marked (MDR, Notified Body number 2797)
  • Canada: Health Canada Licence
  • Brazil: registered
  • Other regions: information available upon request

Product codes

  • FLUSH020 : 5x 20ml FertiCultTM Flushing medium
  • FLUSH050 : 5x 50ml FertiCultTM Flushing medium
  • FLUSH100 : 3x 100ml FertiCultTM Flushing medium
  • FLUSH020PHR : 5x 20ml FertiCultTM Flushing medium with phenol red
  • FLUSH050PHR : 5x 50ml FertiCultTM Flushing medium with phenol red
  • FLUSH100PHR : 3x 100ml FertiCultTM Flushing medium with phenol red
  • FLUSH500PHR : 1x 500ml FertiCultTM Flushing medium with phenol red
  • FLUSH020PRG : 5x 20ml FertiCultTM Flushing medium with phenol red and gentamicin
  • FLUSH050PRG : 5x 50ml FertiCultTM Flushing medium with phenol red and gentamicin
  • FLUSH100PHR_G : 3x 100ml FertiCultTM Flushing medium with phenol red and gentamicin
  • FLUSH500PHR_G: 1x 500ml FertiCultTM Flushing medium with phenol red and gentamicin
pH 7.30-7.90 (7.30-7.60 at product release)
Osmolality 270-290mOsm/kg
Sterility by the current Ph. Eur. 2.6.1 / USP <71> No growth
Endotoxin <0.25 EU/ml
Mouse embryo assay (% blastocysts after 96 hours, after 60 minutes exposure to test medium) ≥ 80%
Human sperm survival assay (% motility compared with control after 24 hours) ≥ 80%
Human serum albumin concentration 3.2-4.8 g/l
Shelf life 18 months from date of production

Sperm ProcessingOocyteMediaEmbryo TransferInsemination

FertiCult Flushing medium is a ready-to-use cell culture medium for washing procedures, swim-up, IUI and embryo transfer

FertiCult_flushing_medium
Instructions For Use
Summary of safety and clinical performance (SSCP)
Material Safety Data Sheet