VitriFreeze ES – VitriThaw ES

VitriFreeze ES^TM – VitriThaw ES^TM

Intended use
VitriFreeze ES™ / VitriThaw ES™ are a set of media for vitrification and thawing of human embryos (zygote till expanded blastocyst stage).

VitriFreeze ES™ / VitriThaw ES™ are ready-to-use.

Composition

VitriFreeze ES™ / VitriThaw ES™ contain PBS and human serum albumin (11-20 g/l depending on the individual medium, medicinal substance derived from human blood plasma). None of the media contain antibiotics. VitriFreeze ES™ media have increasing concentrations of DMSO and ethylene glycol (EG). VitriFreeze ES™ Freezing 3 also contains sucrose and Ficoll. VitriThaw ES™ media have decreasing concentrations of sucrose.

Further information on the composition of the medium can be found in the material safety data sheet.

Regulatory

Europe: CE-marked (MDR, Notified Body number 2797)
Brazil: Registered
Other regions: Information available upon request

Product order codes

VF_KIT1_ES : VitriFreeze ES™ kit
VT_KIT1_ES : VitriThaw ES™ kit

Product specifications

pH	7.2-7.4
Osmolality	VitriFreeze ES™ Pre-incubation medium : 270-295mOsm/kg (release criteria: 270-290mOsm/kg) VitriThaw ES™ Thawing medium 2: 805-865mOsm/kg (release criteria: 805-850mOsm/kg) VitriThaw ES™ Thawing medium 3: 535-565mOsm/kg VitriThaw ES™ Thawing medium 4: 405-435mOsm/kg VitriThaw ES™ Thawing medium 5: 270-295mOsm/kg (release criteria: 270-290mOsm/kg)
Sterility test by the current Ph. Eur. 2.6.1/ USP <71>	No growth
Endotoxin	< 0.25 EU/mL
Mouse embryo assay (% blastocysts after 96 hours, after sequential exposure to freezing and thawing media)	≥ 80%
Shelf life	12 months from date of production