SpermMar Test IgG

Test for the detection of anti-sperm antibodies

SpermMar Test IgA and SpermMar Test IgG are rapid, easy-to-use tests for the detection of anti-sperm antibodies in semen and serum.

Anti sperm antibodies (ASA) are produced when the blood-testis barrier is disrupted by obstruction, infection or traumatism. Immunoglobulines of the IgG class are usually present in both serum and semen, and the IgG antibodies attach to the spermatozoa. ASA of the IgA class are secreted by the accessory sex glands and are found in semen only. ASA of the IgG class may be detected in seminal plasma, but there is usually no free IgA in seminal plasma. Anti sperm antibodies of the IgA class almost never occur without IgG antibodies.

In case more than 40% of motile spermatozoa present ASA of the IgG class, complementary testing for IgA antibodies is advisable.

The immunological factor reduces the probability of spontaneous conception, and this is particularly true when ASA of the IgA class are also present.

SpermMar Test IgG has an 18 month shelf life from production date. Information about the composition of the product can be found in the material safety data sheet.

Regulatory

Europe: CE-marked – USA: registered – Canada: Health Canada license
Brazil: registered – Australia: registered

Product order codes

SPMG_S : SpermMar Test IgG – Single kit – 50 tests
SPMG_C : SpermMar Test IgG – Complete kit – 50 tests
SPMG_P : SpermMar Test IgG – Positive control
SPMG_N : SpermMar Test IgG – Negative control

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Bohring C., Klepper L., Krause W., Localization of binding sites of naturally occurring antisperm antibodies on human spermatozoa by immunofluorescence, Andrologia (2004),Vol.36,pp.286-290

Bohring C., Krause E., Habermann B., Krause W., Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease, Molecular Human Reproduction (2001),Vol.7,No.2,pp.113-118

Bohring C., Krause W., Characterization of Spermatozoa Surface Antigens by Antisperm Antibodies and its Influence on Acrosomal Exocytosis, American Journal of Reproductive Immunology (2003),Vol.50,pp.411-419

Bohring C., Krause W., The characterization of human spermatozoa membrane proteins – surface antigens and immunological infertility, Electrophoresis (1999),Vol.20,pp.971-976

Bohring C., Krause W., The intra- and inter-assay variation of the indirect mixed antiglobulin reaction test: is a quality control suitable?, Human Reproduction (1999),Vol.14,No.7,pp.1802-1805

Bozhedomov V.A., Nikolaeva M.A., Ushakova I.V., et al., Functional deficit of sperm and fertility impairment in men with antisperm antibodies , Journal of Reproductive Immunology (2015),Vol.112,pp.95-101

Chavez-Badiola A., Drakeley A.J., Finney V., et al., Necrospermia, antisperm antibodies, and vasectomy, Fertility and Sterility (2008),Vol.89,No.3,pp.723e5-e7

Chiu W.W-C., Chamley L.W., Human seminal plasma prolactin-inducible protein is an immunoglobulin G-binding protein, Journal of Reproductive Immunology (2003),Vol.60,pp.97-111

Depuydt C.E., Bosmans E., Zalata A., et al., The Relation Between Reactive Oxygen Species and Cytokines in Andrological Patients With or Without Male Accesory Gland Infection, Journal of Andrology (1996),Vol.17,No.6,pp.699-707

Depuydt C.E., De Potter C.R., Zalata A., et al., Levels of Hepatocyte Growth Factor/Scatter Factor (HGF/SF) in Seminal Plasma of Patients With Andrological Diseases, Journal of Andrology (1998),Vol.19,No.2,pp.175-182

Garolla A., Pizzol D., Bertoldo A., et al., Association, prevalence, and clearance of human papillomavirus and antisperm antibodies in infected semen samples from infertile patients, Fertility and Sterility (2013),Vol.99,No.1,pp.125-131

Gatimel N., Moreau J., Isus F., et al., Anti-sperm antibodies detection by a modified MAR test: Towards a better definition of its indications, Reproductive biomedicine online (2018),Vol.37,No.6,pp.717-723

Hadinedoushan H., Ghafourzadeh M., A survey of anti-sperm antibodies in infertile couples, Iranian Journal of Reproductive Medicine (2007),Vol.5,No.1,pp.39-40

Hussein T.M., Elneily D., Eid A.A., Abou-ElKhier H., Assessment of antisperm antibodies in a sample of Egyptian patients with hepatitis C virus infection, Andrologia (2017),Vol.49,No.e12664

Laudat A., Burc-Struxiano L., Is Absence of Spontaneous Agglutinates of Spermatozoa in Semen a Reliable Indicator of Non-Autosensization against Human Sperm Antigens?, Open Access Library Journal (2016),Vol.3,No.e2978

Lotti F., Baldi E., Corona G., et al., Epididymal more than testicular abnormalities are associated with the occurence of antisperm antibodies as evaluated by the MAR test, Human Reproduction (2018),Vol.33,No.8,pp.1417-1429

Marconi M., Nowotny A., Pantke P., et al., Antisperm antibodies detected by mixed agglutination reaction and immunobead test are not associated with chronic inflammation and infection of the seminal tract, Andrologia (2008),Vol.40,pp.227-234

Marconi M., Pilatz A., Wagenlehner F., et al., Are Antisperm Antibodies Really Associated with Proven Chronic Inflammatory and Infectious Diseases of the Male Reproductive Tract?, European Urology (2009),Vol.56,pp.708-715

Ombelet W., Vandeput H., Janssen M., et al., Treatment of male infertility due to sperm surface antibodies: IUI or IVF?, Human Reproduction (1997),Vol.12,No.6,pp.1165-1170

Poppe K., Glinoer D., Tournaye H., et al., Is systematic screening for thyroid disorders indicated in subfertile men?, European Journal of Endocrinology (2006),Vol.154,pp.363-366

Rossato M., Galeazzi C., Ferigo M., Foresta C., Antisperm antibodies modify plasma membrane functional integrity and inhibit osmosensitive calcium influx in human sperm, Human Reproduction (2004),Vol.19,No.8,pp.1816-1820

Tennakoon V., Weerasekera DS., Yasawardene SG., Successful pregnancy outcomes following in-vitro fertilization in infertile couples with high levels of antisperm antibodies, Sri Lanka Journal of Obstetrics and Gynaecology (2010),Vol.32,pp.47-48

Tennakoon V., Yasawardene S.G., Weerasekera D.S., Possible risk factors for the formation of antisperm antibodies in a subfertile population, Sri Lanka Journal of Obstetrics and Gynaecology (2011),Vol.33,pp.12-19

Verón G.L., Molina R.I., Tissera A.D., et al., Incidence of Sperm Surface Autoantibodies and Relationship with Routine Semen Parameters and Sperm Kinematics, Am J Reprod Immunol (2016),Vol.76,pp.59-69

The immunological factor reduces the probability of spontaneous conception, and this is particularly true when ASA of the IgA class are also present

Instructions For Use

Material Safety Data Sheet

SpermMar IgG Test kit
Positive And Negative Controls