Hyaluronidase in FertiCult Flushing medium
80IU hyaluronidase solution in FertiCult Flushing medium
Hyaluronidase in FertiCult Flushing medium contains 80 IU/ml hyaluronidase from bovine origin, in FertiCult Flushing medium (HEPES-buffered medium which contains 4.00g/liter human serum albumin). The product is ready for use. Information about the composition of the product can be found in the material safety data sheet.
Hyaluronidase in FertiCult Flushing medium is used in the oocyte denudation process. Hyaluronidase digests the hyaluronic acid between the cumulus cells, which makes it easier to remove the cumulus mechanically.
Regulatory
Europe: CE-marked – Brazil: registered
Product order codes
HYA001 : Hyaluronidase solution in Flushing Medium – 5x 1 mL
HYA010 : Hyaluronidase solution in Flushing Medium – 10 mL
| pH | 7.3-7.6 |
| Osmolality | 270-290 mOsm/kg |
| Sterility | Sterile |
| Endotoxin | < 1.0 EU/mL |
| Mouse embryo assay | ≥ 80% blastocysts after 96h incubation (30 minute exposure at 1-cell stage) |
| Albumin | FDA (USA) and EMA (Europe) compliant |
| Shelf life | 12 months from date of produce |
| Component | Benefit |
| HEPES (18 mM) | HEPES stabilizes the pH under air (Clark and Swain 2014), therefore CO2 incubation is not required. |
| HSA (4 g/L) |
Hyaluronidase in FertiCult Flushing medium contains 4 g/Liter Human Serum Albumin (HSA) to optimize medium performances. HSA is universally added to most ART media because it is widely considered to be of benefit.
The role of albumin in ART media is extensive, including: |
| Hyaluronidase (80IU/ml) | Hyaluronidase in FertiCult Flushing medium contains bovine derived hyaluronidase (80IU/ml). Hyaluronidase is an enzyme derived from bovine testes and is capable of hydrolyzing hyaluronic acid (Tranchepain, et al. 2006), the latter being one of the most abundant constituents of the extracellular matrix (Laurent and Fraser 1992).
When collected, each oocyte is surrounded by a matrix of cells called the cumulus-oocyte complex (COC). The COC is composed of cumulus granulosa cells embedded in a matrix of long hyaluronan oligosaccharide chains cross-linked by a complex of hyaluronan binding cell surface and extracellular matrix proteins and proteoglycans (Richards 2005). In vivo, enzymes like hyaluronidase are released by the acrosome reaction of the sperm cells and dissolve the intracellular matrix between the cumulus cells. Also in vitro during ART, COC around oocytes are removed (i.e. “oocyte denudation”). For that purpose, the European Society of Human Reproduction and Embryology (ESHRE) recommends to use an enzymatic procedure with hyaluronidase, which facilitates the subsequent mechanical denudation process using a pipette (Magli et al. 2008). |
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Hyaluronidase in FertiCult Flushing medium is a ready-to-use cell culture medium for oocyte denudation
