VitriFreezeTM / VitriThawTM

Intended use

VitriFreeze™ / VitriThaw™ are a set of media for vitrification and thawing of human embryos (morula till expanded blastocyst stage).

VitriFreeze™ and VitriThaw™ are ready to use.


VitriFreeze™ / VitriThaw™ contain PBS and human serum albumin (11-20 g/l depending on the individual medium, medicinal substance derived from human blood plasma). None of the media contain antibiotics. VitriFreeze™ media have increasing concentrations of DMSO and ethylene glycol (EG). VitriFreeze™ Freezing 2 also contains sucrose and Ficoll. VitriThaw™ media have decreasing concentrations of sucrose.

Further information on the composition of the medium can be found in the material safety data sheet.


  • Europe: (CE-marked MDR, Notified Body number 2797)
  • USA: US FDA Cleared
  • Other regions: Information available upon request

Product order codes

VF_KIT1: VitriFreeze™ kit
VF_KIT1: VitriThaw™ kit

pH 7.2-7.4
Osmolality VitriFreeze™ Pre-incubation medium: 270-295mOsm/kg (release criteria: 270-290mOsm/kg)
VitriThaw™ Thawing medium 1: 805-865mOsm/kg (release criteria: 805-850mOsm/kg).
VitriThaw™ Thawing medium 2: 535-565mOsm/kg
VitriThaw™ Thawing medium 3: 405-435mOsm/kg
VitriThaw™ Thawing medium 4: 270-295mOsm/kg (release criteria: 270-295mOsm/kg)
Sterility test by the current Ph. Eur. 2.6.1/ USP <71> No growth
Endotoxin < 0.25 EU/mL
Mouse embryo assay (% blastocysts after 96 hours, after sequential exposure to freezing and thawing media) ≥ 80%
Shelf life 12 months from date of production


VitriFreezeTM and VitriThawTM media are sets of cell culture media designed for the vitrification and thawing of morula up to blastocyst stage human embryos

Instructions For Use
Summary of safety and clinical performance
Material Safety Data Sheet