VitriFreeze – VitriThaw

VitriFreeze^TM / VitriThaw^TM

Intended use

VitriFreeze™ / VitriThaw™ are a set of media for vitrification and thawing of human embryos (morula till expanded blastocyst stage).

VitriFreeze™ and VitriThaw™ are ready to use.

Composition

VitriFreeze™ / VitriThaw™ contain PBS and human serum albumin (11-20 g/l depending on the individual medium, medicinal substance derived from human blood plasma). None of the media contain antibiotics. VitriFreeze™ media have increasing concentrations of DMSO and ethylene glycol (EG). VitriFreeze™ Freezing 2 also contains sucrose and Ficoll. VitriThaw™ media have decreasing concentrations of sucrose.

Further information on the composition of the medium can be found in the material safety data sheet.

Regulatory

Europe: (CE-marked MDR, Notified Body number 2797)
USA: US FDA Cleared
Other regions: Information available upon request

Product order codes

VF_KIT1: VitriFreeze™ kit
VT_KIT1: VitriThaw™ kit

Product specifications

pH	7.2-7.4
Osmolality	VitriFreeze™ Pre-incubation medium: 270-295mOsm/kg (release criteria: 270-290mOsm/kg) VitriThaw™ Thawing medium 1: 805-865mOsm/kg (release criteria: 805-850mOsm/kg). VitriThaw™ Thawing medium 2: 535-565mOsm/kg VitriThaw™ Thawing medium 3: 405-435mOsm/kg VitriThaw™ Thawing medium 4: 270-295mOsm/kg (release criteria: 270-295mOsm/kg)
Sterility test by the current Ph. Eur. 2.6.1/ USP <71>	No growth
Endotoxin	< 0.25 EU/mL
Mouse embryo assay (% blastocysts after 96 hours, after sequential exposure to freezing and thawing media)	≥ 80%
Shelf life	12 months from date of production

Frequently Asked Questions Certificate Of Analysis

Cryopreservation

VitriFreeze^TM and VitriThaw^TM media are sets of cell culture media designed for the vitrification and thawing of morula up to blastocyst stage human embryos

Instructions For Use

Summary of safety and clinical performance

SSCP

VitriFreezeTM / VitriThawTM

VitriFreezeTM and VitriThawTM media are sets of cell culture media designed for the vitrification and thawing of morula up to blastocyst stage human embryos

Instructions For Use

Summary of safety and clinical performance

Material Safety Data Sheet

VitriFreeze^TM / VitriThaw^TM

VitriFreeze^TM and VitriThaw^TM media are sets of cell culture media designed for the vitrification and thawing of morula up to blastocyst stage human embryos