Frequently Asked Questions: FertiCult Flushing medium

FAQ1: Does FertiCult Flushing medium contain phenol red?
We have different “versions” of our FertiCult Flushing medium.
The standard product does not contain phenol red but we also have product formulations which contain the pH marker.

FAQ2: Do I have to add my own antibiotics to the FertiCult Flushing Medium ?
We have product available both with and without added antibiotics (gentamicin). If you prefer to add your own antibiotics we suggest to add 10mg of gentamicin per liter of medium.

FAQ3: Can I use FertiCult Flushing medium to wash sperm and do sperm swim-ups ?
Yes you can, FertiCult Flushing medium is HEPES buffered and can therefore be used at room temperature or at body temperature without the need for an CO2 incubator. The medium has been very strictly tested on human sperm, mouse embryo’s, endotoxine levels (<0.25 EU/ml), osmolality, pH and sterility and is therefore save for use in any swim-up or swim-down procedure.

FAQ4: Can I use FertiCult Flushing medium for oocyte retrieval ?
No, you could use FertiCult Aspiration medium which doesn’t require any additives.

FAQ5: Can I use FertiCult Flushing medium for ICSI ?
Yes you can, just make sure to keep the medium as close to body temperature as possible during the procedure.

FAQ6: Should we pre-incubate FertiCult Flushing medium prior to use ?
You should bring the FertiCult Flushing medium to the correct temperature before use. Never put FertiCult Flushing medium in a CO2 incubator since this will lower the pH to approximately 6.90-6.95.

FAQ7: How long can I use FertiCult Flushing medium after opening the bottle?
2 factors limit the shelf life of a product after opening the bottle:

  • Gass exchange: influences the pH
  • Contamination with viable organisms

We have performed open bottle tests on our media for up to 7 days and found that the product parameters stayed within the specifications in this time period, taking into consideration that the bottle was opened under sterile conditions and the product was stored at 2-8°C after opening.

FAQ8: Is there a difference between FertiCult Flushing medium and Sperm Washing/Insemination medium?
No, both products are identical. When FertiCult Flushing medium is assembled in a kit together with Sil-Select Plus media, the products are marketed under the name “Sil-Select Plus Sperm Washing/Insemination media”.

FAQ9: Which volume of semen should be used for swim-up?
This depends on the type of tube. If Falcon tubes of 15ml are used (which is recommended): use 1ml semen, and overlay with 1.5ml FertiCult Flushing medium. This way the surface between both layers can be maximized, leading to better yields. If the semen sample has a higher volume, use more tubes or wash the semen before swim-up to resuspend in a lower volume. Also, if a lot of debris is present, more tubes are recommended.

FAQ10: Is it normal that the medium becomes a bit acidic upon incubation at 37°C?
This is normal, pH decreases with 0.10 if the medium is placed at 37°C compared to 22°C.