FAQ1: Should the whole bottle be prewarmed at 37°C?
No, prepare dishes and place these in the CO2 incubator at 37°C. The vial with remaining medium should be stored at 2-8°C as soon as possible.
FAQ2: Can GAIN medium be used to incubate raw (mature) oocytes?
Yes, it can.
FAQ3: Does altitude have an influence on the required CO2 settings?
Yes, because the atmospheric air pressure changes. The higher the center is located, the higher the CO2 settings should be. The CO2 settings are more of a guideline to achieve an optimal pH, because it is very important to have the right pH.