Frequently Asked Questions: FertiCultTM IVF medium
FAQ: FAQ1: Do I have to add my own antibiotics to FertiCult™ IVF Medium ?
No, we have product variants available which contain 10 mg/l gentamicin.
FAQ2: Should I pre-incubate FertiCult™ IVF medium before use ?
Yes, FertiCult™ IVF medium has to be preincubated in a CO2 incubator with 5-6% CO2 for at least 5 hours. Ideally, dishes or tubes should be prepared the day before use and incubated overnight (with lid opened) to obtain an optimal pH for oocyte washing/holding.
FAQ3: For how many days of culture can we use FertiCult™ IVF medium ?
It is designed for short term culture only (until 2PN). Afterwards, the zygote can be placed in an embryo culture medium of choice (e.g. GAIN™ medium, FertiPro NV).
FAQ4: Should the whole bottle be prewarmed at 37°C? How long can I use FertiCult™ IVF medium after opening the bottle?
No, ideally, prepare dishes one day beforehand and place these in the CO2 incubator at 37°C. The bottle with remaining medium should be stored at 2-8°C as soon as possible.
We have performed in-use testing on FertiCult™ IVF medium which supports the use of the media up to 7 days, taking into account that the bottle was opened under sterile conditions and the product was stored at 2-8°C after opening. In addition and as indicated in the IFU it is advised to:
- keep the product in its original container until the day of use and
- depending on the number of procedures that will be performed on one day, remove the required volume of medium under aseptic conditions in an appropriate sterile recipient. Only pre-warm sterile recipient. This is in order to avoid multiple openings/warming cycles of the medium. Discard excess (unused) media.