Frequently Asked Questions: FertiCultTM IVF medium

FAQ: FAQ1: Do I have to add my own antibiotics to FertiCult IVF Medium ?

No, we have product variants available which contain 10 mg/l gentamicin.

FAQ2: Should I pre-incubate FertiCult IVF medium before use ?

Yes, FertiCult IVF medium has to be preincubated in a CO2 incubator with 5-6% CO2 for at least 5 hours. Ideally, dishes or tubes should be prepared the day before use and incubated overnight (with lid opened) to obtain an optimal pH for oocyte washing/holding.

FAQ3: For how many days of culture can we use FertiCult IVF medium ?

It is designed for short term culture only (until 2PN). Afterwards, the zygote can be placed in an embryo culture medium of choice (e.g. GAINmedium, FertiPro NV).

FAQ4: Should the whole bottle be prewarmed at 37°C? How long can I use FertiCult IVF medium after opening the bottle?

No, ideally, prepare dishes one day beforehand and place these in the CO2 incubator at 37°C. The bottle with remaining medium should be stored at 2-8°C as soon as possible.

We have performed in-use testing on FertiCult IVF medium which supports the use of the media up to 7 days, taking into account that the bottle was opened under sterile conditions and the product was stored at 2-8°C after opening. In addition and as indicated in the IFU it is advised to:

  • keep the product in its original container until the day of use and
  • depending on the number of procedures that will be performed on one day, remove the required volume of medium under aseptic conditions in an appropriate sterile recipient. Only pre-warm sterile recipient. This is in order to avoid multiple openings/warming cycles of the medium. Discard excess (unused) media.