Frequently Asked Questions: LeucoScreen Plus

FAQ1: How is the concentration of round cells calculated?
Count the number of round cells whilst determining the sperm concentration in a routine semen analysis. If the concentration of round cells exceeds 1×106 cells/ml, additional testing with LeucoScreen Plus is recommended.

FAQ2: What are the advantages of the LeucoScreen Plus kit compared to the LeucoScreen kit?
The LeucoScreen Plus kit is equally suitable to distinguish between peroxidase-positive and peroxidase-negative round cells, but has the major advantage of not containing a carcinogenic substrate when compared with the LeucoScreen kit.

FAQ3: For how many tests can the kit be used?
There is enough reagent to create the work solution 20 times. The work solution is stable for 2 days, so the kit contains reagents for 40 days of testing.

FAQ4: How to evaluate peroxidase-positive versus -negative round cells?
A demonstration video is available on the LeucoScreen Plus product page. A training is available upon request.

FAQ5: Can we perform the LeucoScreen Plus test in case of very high or low round cell concentrations in the sample?
In some semen samples, you might prefer to enrich the round cell concentration to facilitate cell counting. Therefore, centrifuge the sample for 15 minutes at 350g, remove some volume of the semen sample and resuspend the pellet.

In case of very high concentration of round cells (i.e. above 20×106 per ml), it is strongly advised to dilute the sample in PBS or in FertiCultTM Flushing medium.