FAQ1: What is the origin of the antibodies and other biological material?
The SpermMar Test IgA contains bovine sourced albumin. The beads are coated with rat anti-human IgA antibodies.
FAQ2: How long can we wait before the test should be performed?
Perform the test preferably within 1h after collection, especially in samples with low motility. If motility is high, it might be possible to perform the test later (max 3-4h later).
FAQ3: Can or should we do an indirect SpermMar IgA test ?
No, you probably shouldn’t.
Performance of the indirect IgA SpermMar test is to be considered experimental and the clinical meaning of its results has not been established.
FAQ4: Can we perform the SpermMar IgA test on washed spermcells ?
We advice against washing the spermcells before performing the direct SpermMar IgA test for a number of reasons.
First of all, some washing media contain human serum albumin (HSA). HSA tends to form a “shield” around the spermcells which hide the antibodies so that they can not be detected by the test method.
Secondly, washing the sperm sample will remove most of the antibodies that could be present in the seminal plasma and might thus render false negative results.
FAQ5: We do the SpermMar IgG test, should we also do the SpermMar IgA test ?
Some laboratories only do the SpermMar IgG on a routine basis, in case of a positive IgG they also do an IgA. It does happen however that a patient with a negative IgG is positive for IgA.
So, from a clinical point of view you should do both SpermMar IgG and SpermMar IgA, from an economical point of view however you would probably opt for the IgG as a routine test and IgA for positive IgG’s.
FAQ6: Why does the SpermMar IgG kit contain 2 bottles and the SpermMar IgA only 1 ?
The SpermMar IgG kit contains one bottle of latex particles coated with IgG and one bottle of anti-IgG antiserum. The antiserum actually links the antibodies on the surface of the spermatozoa to the antibodies on the surface of the latex particles.
The SpermMar IgA on the other hand contains only one bottle of latex particles coated with monoclonal anti-IgA directly linking the latex particles to the antibodies on the surface of the spermatozoa. The SpermMar IgA therefore only needs 1 bottle of reagents where the SpermMar IgG needs 2.
FAQ7: Do you supply positive or negative controls for the SpermMar IgA test ?
No we don’t. Because the SpermMar IgA test can only be used directly on sperm, it is impossible for us to ship motile spermatozoa. The positive and negative controls for the SpermMar IgG test contain plasma and hence can be stored for long time periods.
FAQ8: What is the difference between the single kit and the complete kit?
The single kit only contains the reagents, while the complete kit also contains the lab disposables (micro slides, cover glasses, microcapillary pipettes with rubber bulb).
FAQ9: Can the test be done on frozen/thawed semen samples?
No, it is recommended on native samples because HSA in cryoprotectant can mask anti-sperm antibodies on spermatozoa.
FAQ10: What is the shelf life after opening?
If the product is used aseptically, firmly closed and kept refrigerated between tests, the shelf life after opening remains the same as when unopened (12 months).
FAQ11: What are the minimal spermatozoa parameters needed to perform a SpermMar Test IgA?
A concentration of >10 million/ml and a progressive motility of >30 % is advisable. The sperm samples can be concentrated or diluted, but it is advisable not to use a medium containing HSA or another protein supplement as this can interfere with the test results.
FAQ12: When is it recommended to perform a quality control? How do we perform quality control on the SpermMar Test IgA?
This decision is lab-dependent. Most labs do a quality control when starting a new lot. It is possible to compare results of a known positive and a known negative donor, tested with the kit in use and a new kit.