FertiCult Flushing medium

FertiCult^TM Flushing medium

Intended use

FertiCult^TM Flushing medium is intended for in vitro procedures involving human gametes (sperm and oocytes), including washing of gametes, sperm swim-up procedures, intra-uterine insemination (IUI) of the spermatozoa and sperm injection during intracytoplasmic sperm injection (ICSI). FertiCult^TM Flushing medium can also be used for human embryo washing and holding, and for embryo transfer (ET) in the uterine cavity.

FertiCult^TM Flushig medium is ready-to-use.

Composition
FertiCult^TM Flushing medium is a ready-to-use HEPES buffered medium which also contains bicarbonate, physiologic salts, glucose, lactate, pyruvate and human serum albumin (4.0g/l, medicinal substance derived from human blood plasma). The product is also available with phenol red and/or gentamicin (10mg/l, medicinal substance).

Further information on the composition of the media can be found in the material safety data sheet.

Regulatory

Europe: CE marked (MDR, Notified Body number 2797)
Canada: Health Canada Licence
Brazil: registered
Other regions: information available upon request

Product codes

FLUSH020 : 5x 20ml FertiCult^TM Flushing medium
FLUSH050 : 5x 50ml FertiCult^TM Flushing medium
FLUSH100 : 3x 100ml FertiCult^TM Flushing medium

FLUSH020PHR : 5x 20ml FertiCult^TM Flushing medium with phenol red
FLUSH050PHR : 5x 50ml FertiCult^TM Flushing medium with phenol red
FLUSH100PHR : 3x 100ml FertiCult^TM Flushing medium with phenol red

FLUSH500PHR : 1x 500ml FertiCult^TM Flushing medium with phenol red

FLUSH020PRG : 5x 20ml FertiCult^TM Flushing medium with phenol red and gentamicin
FLUSH050PRG : 5x 50ml FertiCult^TM Flushing medium with phenol red and gentamicin
FLUSH100PHR_G : 3x 100ml FertiCult^TM Flushing medium with phenol red and gentamicin

FLUSH500PHR_G: 1x 500ml FertiCult^TM Flushing medium with phenol red and gentamicin
WASH005: 25x 5ml Sil-Select Plus^TM Sperm Washing/Insemination medium
WASH020: 5x 20ml Sil-Select Plus^TM Sperm Washing/Insemination medium
WASH100G: 1x 100ml Sil-Select Plus^TM Sperm Washing/Insemination medium with gentamicin

Product specifications

pH	7.30-7.90 (7.30-7.60 at product release)
Osmolality	270-290mOsm/kg
Sterility by the current Ph. Eur. 2.6.1 / USP <71>	No growth
Endotoxin	<0.25 EU/ml
Mouse embryo assay (% blastocysts after 96 hours, after 60 minutes exposure to test medium)	≥ 80%
Human sperm survival assay (% motility compared with control after 24 hours)	≥ 80%
Human serum albumin concentration	3.2-4.8 g/l
Shelf life	18 months from date of production