GAIN medium – FertiPro

GAIN^TM medium

Intended use:

GAIN^TM medium is a single-step cell culture medium for use with human embryos and gametes. GAIN™ medium can be used in all the procedures below:

For semen washing and intra uterine insemination (IUI).
For oocytes handling/incubation in preparation of, or during fertilization by in vitro fertilization (IVF)/ intra cytoplasmatic sperm injection (ICSI).
For embryo culture from day 1 to expanded blastocyst stage.
For embryo transfer (ET).

GAIN^TM medium is complete and needs no further additives.

Composition

GAIN^TM medium is a bicarbonate-buffered balanced salt solution, supplemented with 10mg/l gentamicin (medicinal substance) and 3.5g/l human serum albumin (medicinal substance derived from human blood plasma) and phenol red.

Further information on the composition of the medium can be found in the material safety data sheet.

Regulatory:

Europe: CE-marked (MDR, Notified Body number 2797)
Brazil: Registered
Other regions: Information available upon request

Product order codes:

GAIN010 : 5 x 10ml – GAIN^TM medium
GAIN020 : 5 x 20ml – GAIN^TM medium

Product specifications

pH	7.20-7.45 (pH 37°C – 5% CO₂)
Osmolality	270-290mOsm/kg
Sterility by the current Ph. Eur. 2.6.1./ USP <71>	No growth
Endotoxin	< 0.25 EU/mL
Mouse embryo assay (% blastocysts after 96 hours culture in test medium)	≥ 80%
Human sperm survival assay (% motility compared with control after 24 hours exposure to test medium)	≥ 80%
Concentration human serum albumin	2.8-4.2 g/l
Shelf life	12 months from date of production