### Frequently Asked Questions: Fructose Test

FAQ1: Can the Fructose test be performed on frozen/thawed semen plasma samples?
Yes it can. It is best to centrifuge the semen sample before freezing the semen plasma at -18°C or below.

FAQ2: Is measurement at alternative wavelengths possible?
It is recommended to measure at 450-492nm, no data on other wavelengths is available. If an alternative wavelength is applied, investigate the efficiency before implementing it.

FAQ3: Is there a template for the calculations available?
Yes, it can be downloaded from our website in the product page. Alternatively, an Excel-sheet template can be downloaded directly by clicking here.

FAQ4: Calculation of g-forces
The g-force of your centrifuge can be calculated using this formula:
g = 1.118 x r x rpm² or rpm = Square root {g / (1.118 x r)}
r = radius of centrifuge in mm; rpm = rotations per minute / 1000
e.g.1
r = 100 mm rpm = 3000 rotations per minute
g = 1.118 x 100 x 9 = 1006g
e.g.2
r = 100 mm g = 1200g
rpm = SQR {1200 / (1.118 x 100)} = 3.28
= 3280 rotations per minute

Measuring range of the Fructose Test is defined as 0.5-5 mg/ml. If higher concentrations are determined it is advised to repeat the test with diluted sample in FertiCult Flushing medium (FertiPro NV).