Frequently Asked Questions: SpermFreeze SSP

FAQ1: Does SpermFreeze SSP contain antibiotics?
No, SpermFreeze SSP does not contain any antibiotics.

FAQ2: Do I have to wash thawed samples before use?
We strongly suggest washing the sperm sample after thawing to remove the glycerol from the medium.

FAQ3: How long can I keep sperm in a frozen state?
Once frozen at -196°C, sperm can be kept for 10 to 20 years (possibly even longer). The fertilising potential can be diminished after a long period of freezing.
The critical part in the freezing process is the actual freezing itself, this is where the damage is done to the cells. SpermFreeze SSP was especially designed to avoid damage during freezing, it is advisable however to test-freeze a semen sample. If viability and motility are dramatically decreased, try a programmed freezing protocol.

FAQ4: Do I need to add milk or egg-yolk to SpermFreeze SSP?
No you don’t!
SpermFreeze SSP is a ready to use freezing medium which doesn’t require any additives. Just follow the instructions and the sperm sample will be safely frozen and thawed.

FAQ5: How long can I use SpermFreeze SSP after opening the bottle?
Two factors limit the shelf life of a product after opening the bottle:

  • Gass exchange: influences the pH
  • Contamination with viable organisms

We have performed open bottle tests on our media for up to 7 days and found that the product parameters stayed within the specifications in this time period, taking into consideration that the bottle was opened under sterile conditions and the product was stored at 2-8°C after opening.

FAQ6: Why place an air bubble in the middle of the straw?
To prevent rupture of the seal at the end of the straw when SpermFreeze SSP/semen mix expands during freezing.

FAQ7: Is SpermFreeze SSP IUI-ready?
No, SpermFreeze SSP medium must be washed off before IUI is performed to avoid transfer of SpermFreeze SSP medium to the uterus.

FAQ8: What should be the temperature of the thawing procedure (tap water)?
The temperature is not so critical. Thawing at 4°C, 20°C or 37°C does not make a difference.

FAQ9: Can a sample be washed before freezing?
Yes, as long as the same dilution factor is used as with fresh semen.

FAQ10: Can a sample be purified before freezing? (gradient or swim-up)
Yes, it can. It is important to keep the same dilution factor as with native semen.

FAQ11: Can SpermFreeze SSP also be used with cryotubes instead of straws?
Yes, the same protocol as mentioned in the IFU of SpermFreeze SSP can be applied.

FAQ12: What is the difference between SpermFreeze and SpermFreeze SSP?
SpermFreeze SSP has a higher glycerol concentration (27% vs 15% in SpermFreeze), which results in less dilution (dilution factor SpermFreeze: 1 part semen + 0.7 parts SpermFreeze; dilution factor SpermFreeze SSP: 3 parts semen + 1 part SpermFreeze SSP).
This reduces the number of straws per semen sample for SpermFreeze SSP, but keeps the same end concentration of glycerol as SpermFreeze.
There is no difference in thawing procedure.