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Frequently asked questions for: Sil-Select™ Plus

FAQ1: Does Sil-Select Plus contain antibiotics ?
No it doesn't.
if you want to add your own antibiotics we suggest to add 10mg of pharmaceutical grade gentamicin per liter of medium.

FAQ2: How long can I use Sil-Select Plus after opening the bottle?
2 factors limit the shelf life of a product after opening the bottle:

  • Gass exchange: influences the pH
  • Contamination with viable organisms

  • We have performed open bottle tests on our media for up to 7 days and found that the product parameters stayed within the specifications in this time period, taking into consideration that the bottle was opened under sterile conditions and the product was stored at 2-8°C after opening.

    FAQ3: Can we make our own gradients instead of using your upper and lower layer density gradients ?
    Yes you can, for that you would need to order Sil-Select Stock instead of Sil-Select Plus.
    Note however that Sil-Select Plus gradients have been optimized for maximum efficiency in the selection of spermatozoa.

    FAQ4: What is the advantage of the inclusion of Human Serum Albumin in the formulation of SIP?
    Inclusion of HSA reduces the unintended adhesion of heavily moving spermatozoa to glass ware.

    FAQ5: How to handle a bad semen sample with a volume >2.5ml?
    Use more than one tube, with a max of 100x106 cells, where the ideal volume ratio of upper layer/lower layer/semen isn't altered (2.5ml each). The gradient/semen can be centrifuged for an additional 5-10 minutes (total of 23-28 min @ 400g). Sil-Select 80% might be used intead of Sil-Select Plus Lower Layer (more sperm will pass through the layers).

    FAQ6: How long after liquefaction can a gradient be performed?
    A gradient should be performed as soon as possible.

    FAQ7: How to resuspend the pellet after centrifugation?
    A syringe or micropipet can be used. Vortex should be avoided because this can cause harm to spermatozoa.

    FAQ8: Which medium should be used for washing after performing density gradient?
    FertiCult Flushing medium or FertiCult Washing/Insemination medium.

    Resources

    Click on the links below for more information.