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Frequently asked questions for: SpermFreeze™ SSP

FAQ1: Does SpermFreeze SSP contain antibiotics?
No, SpermFreeze SSP does not include any antibiotics.
If you feel that antibiotics are need, we suggest to add 10mg/liter of pharmaceutical grade gentamicin to the product.

FAQ2: Do I have to wash thawed samples before use?
We strongly suggest washing the sperm sample after thawing to remove the glycerol from the medium.

FAQ3: How long can I keep sperm in a frozen state?
Once frozen at -196°C, sperm can be kept for 10 to 20 years (possibly even longer). The fertilising potential can be diminished after a long period of freezing.
The critical part in the freezing process is the actual freezing itself, this is where the damage is done to the cells. SpermFreeze was especially designed to avoid damage during freezing, it is advisable however to test-freeze a semen sample. If viability and motility are dramatically decreased, try a programmed freezing protocol.

FAQ4: Do I need to add milk or egg-yolk to SpermFreeze SSP™?
No you don't !
SpermFreeze SSP™ is a ready to use freezing medium which doesn't require any additives. Just follow the instructions and the sperm sample will be safely frozen and thawed.

FAQ5: How long can I use SpermFreeze SSP after opening the bottle?
2 factors limit the shelf life of a product after opening the bottle:

  • Gass exchange: influences the pH
  • Contamination with viable organisms

  • We have performed open bottle tests on our media for up to 7 days and found that the product parameters stayed within the specifications in this time period, taking into consideration that the bottle was opened under sterile conditions and the product was stored at 2-8°C after opening.

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